anti tcf4 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti tcf4
Anti Tcf4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 2512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tcf4/beta-Catenin+XP+Rabbit+mAb/pmc13012068-63-108-111
Average 96 stars, based on 2512 article reviews

anti tcf4 - by Bioz Stars, 2026-10

96/100 stars

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Cell-Signaling:

Article Title: Tcf4 regulates secretory cell fate decisions in the small intestine and colon tumors: insights from transcriptomic, histological, and microbiome analyses.
Article Snippet: .. Primary antibodies: anti-Alpi (rabbit polyclonal, PA522,210, Thermo Fisher Scientific); anti-ChgA (rabbit polyclonal, ab15160, Abcam, Cambridge, UK); anticleaved Casp3 (rabbit monoclonal, #9664, Cell Signaling Technology, Danvers, MA, USA); anti-Krt20 (mouse monoclonal, M7019, Agilent Dako, Santa Clara, CA, USA); anti-Lysozyme (rabbit polyclonal, A0099, Agilent Dako); anti-Muc2 (rabbit polyclonal, sc-15334, Santa Cruz Biotechnology, Dallas, TX, USA); anti-Olfm4 (rabbit monoclonal, #39,141, Cell Signaling Technology); anti-PCNA (rabbit polyclonal, ab18197, Abcam); antiPCNA (mouse monoclonal, ab29, Abcam); anti-Pdgfra (goat polyclonal, AF1062, R&D Systems, Minneapolis, MN, USA); anti-Reg3b (sheep polyclonal, AF5110, R&D Systems); anti-RFP (rabbit polyclonal, 600–401- 379, Rockland, Pottstown, PA, USA); anti-RFP (mouse monoclonal, MA5-15,257, Thermo Fisher Scientific); anti-Tacstd2 (rabbit monoclonal, ab214488, Abcam); anti-Tcf4 (rabbit monoclonal, #2569, Cell Signaling Technology); anti-Tcf4 (rabbit monoclonal, MA5-35,295, Thermo Fisher Scientific). .. Secondary antibodies (all from Thermo Fisher Scientific): goat anti-rabbit IgG (H + L) Alexa Fluor 488 (A11034); goat anti-rabbit IgG (H + L) Alexa Fluor Plus 594 (A32740); goat anti-mouse IgG (H + L) Alexa Fluor 488 (A11001); goat anti-mouse IgG (H + L) Alexa Fluor 594 (A11005); donkey anti-goat IgG (H + L) Alexa Fluor 488 (A11055); donkey antisheep IgG (H + L) Alexa Fluor 488 (A11015).

Article Title: Tcf4 regulates secretory cell fate decisions in the small intestine and colon tumors: insights from transcriptomic, histological, and microbiome analyses
Article Snippet: .. Primary antibodies: anti-Alpi (rabbit polyclonal, PA5-22,210, Thermo Fisher Scientific); anti-ChgA (rabbit polyclonal, ab15160, Abcam, Cambridge, UK); anti-cleaved Casp3 (rabbit monoclonal, #9664, Cell Signaling Technology, Danvers, MA, USA); anti-Krt20 (mouse monoclonal, M7019, Agilent Dako, Santa Clara, CA, USA); anti-Lysozyme (rabbit polyclonal, A0099, Agilent Dako); anti-Muc2 (rabbit polyclonal, sc-15334, Santa Cruz Biotechnology, Dallas, TX, USA); anti-Olfm4 (rabbit monoclonal, #39,141, Cell Signaling Technology); anti-PCNA (rabbit polyclonal, ab18197, Abcam); anti-PCNA (mouse monoclonal, ab29, Abcam); anti-Pdgfra (goat polyclonal, AF1062, R&D Systems, Minneapolis, MN, USA); anti-Reg3b (sheep polyclonal, AF5110, R&D Systems); anti-RFP (rabbit polyclonal, 600–401-379, Rockland, Pottstown, PA, USA); anti-RFP (mouse monoclonal, MA5-15,257, Thermo Fisher Scientific); anti-Tacstd2 (rabbit monoclonal, ab214488, Abcam); anti-Tcf4 (rabbit monoclonal, #2569, Cell Signaling Technology); anti-Tcf4 (rabbit monoclonal, MA5-35,295, Thermo Fisher Scientific). .. Secondary antibodies (all from Thermo Fisher Scientific): goat anti-rabbit IgG (H + L) Alexa Fluor 488 (A11034); goat anti-rabbit IgG (H + L) Alexa Fluor Plus 594 (A32740); goat anti-mouse IgG (H + L) Alexa Fluor 488 (A11001); goat anti-mouse IgG (H + L) Alexa Fluor 594 (A11005); donkey anti-goat IgG (H + L) Alexa Fluor 488 (A11055); donkey anti-sheep IgG (H + L) Alexa Fluor 488 (A11015).

Co-Immunoprecipitation Assay:

Article Title: Bisdemethoxycurcumin suppresses liver fibrosis-associated hepatocellular carcinoma via inhibiting CXCL12-induced macrophage polarization
Article Snippet: Chronic, unresolved inflammation correlates with persistent hepatic injury and fibrosis, ultimately progressing to hepatocellular carcinoma (HCC).. Bisdemethoxycurcumin (BDMC) demonstrates therapeutic potential against HCC, yet its mechanism in preventing hepatic "inflammation-carcinoma transformation" remains incompletely understood.. In the current research, clinical HCC specimens underwent analysis using hematoxylin-eosin (H&E) staining and immunohistochemistry (IHC) to evaluate the expression of fibrosis markers, M2 macrophage markers, and CXCL12.

Lysis:

Incubation:

Article Title: Reduced LHFPL3-AS2 lncRNA expression is linked to altered epithelial polarity and proliferation, and to ileal ulceration in Crohn disease
Article Snippet: Equal amounts of protein, as determined by the Bradford assay (B6916, Sigma), were resolved by electrophoresis in SDS 10% polyacrylamide gel and then transferred to a cellulose nitrate membrane (10401383, Tamar). .. The membrane was incubated with one of the following primary antibodies: anti-TCF4 (#2569 Cell Signaling); anti-CTNNB1 (ab32572 Abcam); anti-CDH1 (#9782 Cell signaling); anti-CDH11 (ab151446 Abcam); anti-JAM-A (sc-53623 Santa-Cruz); antiZO-1 (#33-9100 Thermo Fisher); anti-CCNE1 (ab33911 Abcam); anti-CDK2 (sc-163 Santa-Cruz); anti-GAPDH (#2118 Cell signaling). .. Binding of the primary antibody was detected using an enhanced chemiluminescence kit (XLS142,0250, Cyanagen).

Negative Control:

Chromatin Immunoprecipitation:

Article Title: A niche-dependent redox rheostat regulates epithelial stem cell fate
Article Snippet: .. The following antibodies were used for each ChIP experiment based on instructions: 1:50 dilution anti-HIF1α (Novus Biologicals, NB100-134), 1:50 dilution anti-TCF4 (CST, 2569 T). .. 40 μl of Protein G Dynabeads (Invitrogen, 10003D) were used for each ChIP experiment.

Western Blot:

Article Title: Antimicrobial agent chloroxylenol targets β-catenin-mediated Wnt signaling and exerts anticancer activity in colorectal cancer
Article Snippet: Equal amounts of 20-40 μ g per lane proteins were then separated using SDS-PAGE on an 8% SDS-polyacrylamide gel and transferred to PVDF (MilliporeSigma). .. Western blotting was performed at 4°C overnight with primary antibodies as follows: Anti-V5 (1:1,000; cat. no. 13202S; Cell Signaling Technology, Inc.), anti-Flag (1:1,000; cat. no. 14793S; Cell Signaling Technology, Inc.), anti-non-phospho active β-catenin (1:2,000; cat. no. 8814; Cell Signaling Technology, Inc.), anti-TCF4 (1:2,000; cat. no. 2569; Cell Signaling Technology, Inc.), anti-LGR5 (1:1,000; cat. no. ab273092; Abcam), anti-SOX2 (1:1,000; cat. no. 23064S; Cell Signaling Technology, Inc.), anti-β-catenin (1:2,000; cat. no. Sc-7963; Santa Cruz Biotechnology, Inc.), anti-mLGR5 (1:1,000; cat. no. DF2816; Affinity Biosciences, Ltd.), anti-GAPDH (1:5,000; cat. no. 60004-1-Ig; Proteintech Group, Inc.) and anti-β-actin (1:5,000; cat. no. HC201-01; TransGen Biotech Co., Ltd.). .. Blocking was performed before incubation with primary antibody using 5% non-fat powdered milk (Sangon Biotech Co., Ltd.) at room temperature for 1 h. The washing solution was prepared by adding 0.1% Tween 20 to TBS solution.

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